Methods - Revision history

Mdawson: /* Safety information */

2014-09-01T04:49:47Z

Safety information

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	<br><br><br><br><br><br><br><br><br><br><br>		<br><br><br><br><br><br><br><br><br><br><br>

			<br><br><br>
	=== Safety information ===		=== Safety information ===

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	Jellyfish stings can be at best uncomfortable and at worst lethal. Jellyfish must be handled with the utmost care, and protective clothing and gloves worn at all times. If you have any doubts, do not touch them. Ask for expert advice.		Jellyfish stings can be at best uncomfortable and at worst lethal. Jellyfish must be handled with the utmost care, and protective clothing and gloves worn at all times. If you have any doubts, do not touch them. Ask for expert advice.

	<br>		<br>

	= DNA purification =		= DNA purification =

Mdawson at 04:49, 1 September 2014

2014-09-01T04:49:20Z

← Older revision		Revision as of 21:49, 31 August 2014
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	[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]] <br>		[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]] <br>
	<br>		<br><br><br><br><br><br><br><br><br><br><br>

	=== Safety information ===		=== Safety information ===

Mdawson at 04:48, 1 September 2014

2014-09-01T04:48:16Z

← Older revision		Revision as of 21:48, 31 August 2014
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	[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]] <br>		[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]] <br>
			<br>

	=== Safety information ===		=== Safety information ===

Mdawson at 04:47, 1 September 2014

2014-09-01T04:47:05Z

← Older revision		Revision as of 21:47, 31 August 2014
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	''(From: Dawson et al. 1998)''		''(From: Dawson et al. 1998)''

	=== Equipment~~<br>~~ ===		=== Equipment ===

	1. Sterile syringes, forceps, dissecting scissors, or razor blades<br>2. A way of cleaning the sampling tools if they are not disposable (e.g. flaming metal objects after dipping in ethanol, or a good rinse and wipe dry)<br>3. Vials containing 1-2 ml of preservative (DMSO+NaCl or ethanol [70% to 95%; >90% preferred]; will be provided upon request).<br>4. Chest of ice (in the field); ice, refrigerator, or freezer (at lab/home, separate from food).<br>5. Protective gloves (latex, rubber).<br>6. Waterproof paper.<br>7. Plastic bags and/or jars.<br>8. Plastic tray or container.		<br>1. Sterile syringes, forceps, dissecting scissors, or razor blades<br>2. A way of cleaning the sampling tools if they are not disposable (e.g. flaming metal objects after dipping in ethanol, or a good rinse and wipe dry)<br>3. Vials containing 1-2 ml of preservative (DMSO+NaCl or ethanol [70% to 95%; >90% preferred]; will be provided upon request).<br>4. Chest of ice (in the field); ice, refrigerator, or freezer (at lab/home, separate from food).<br>5. Protective gloves (latex, rubber).<br>6. Waterproof paper.<br>7. Plastic bags and/or jars.<br>8. Plastic tray or container.

	=== Procedure A ~~<br>~~ ===		=== Procedure A ===

	Sampling gut or gonad:<br>1. Collect medusae.<br>2. Starve the medusae for as long as possible.<br>3. Using a clean syringe, flush the guts with tapwater. Repeat several times to displace any debris from the guts.<br>4. Using the same syringe (or other tool), suck up (tear or cut) about 0.1-0.2 ml of gastric and/or gonadal tissues.<br>5. Preserve the tissue in one vial of preservative. (Make sure there is excess preservative; guard against diluting the preservative with too much water.)<br>6. Label the vial and put it on ice. Store on ice, in a freezer or refrigerator until the samples are shipped or analyzed (separate from food).<br>7. Clean tools and gloves, repeat for the next sample.		<br>Sampling gut or gonad:<br>1. Collect medusae.<br>2. Starve the medusae for as long as possible.<br>3. Using a clean syringe, flush the guts with tapwater. Repeat several times to displace any debris from the guts.<br>4. Using the same syringe (or other tool), suck up (tear or cut) about 0.1-0.2 ml of gastric and/or gonadal tissues.<br>5. Preserve the tissue in one vial of preservative. (Make sure there is excess preservative; guard against diluting the preservative with too much water.)<br>6. Label the vial and put it on ice. Store on ice, in a freezer or refrigerator until the samples are shipped or analyzed (separate from food).<br>7. Clean tools and gloves, repeat for the next sample.

	=== Procedure B ~~<br>~~ ===		=== Procedure B ===

	(quicker alternative) - sampling oral arms or bell margin:<br>		<br>(quicker alternative) - sampling oral arms or bell margin:<br>

	1. Collect medusae, place it in a pail. Some scyphozoans are very fragile and so most care must be taken in handling them both before and after fixation to prevent damage. In general, this means keeping them suspended in liquid at all times and never handling them out of the water. Use a plastic tray or container to place the medusae.<br> <gallery widths=150px perrow=7>		1. Collect medusae, place it in a pail. Some scyphozoans are very fragile and so most care must be taken in handling them both before and after fixation to prevent damage. In general, this means keeping them suspended in liquid at all times and never handling them out of the water. Use a plastic tray or container to place the medusae.<br> <gallery widths=150px perrow=7>
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	Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>		Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>

	[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]]		[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]] <br>


	=== Safety information ===		=== Safety information ===

Mdawson at 04:45, 1 September 2014

2014-09-01T04:45:42Z

← Older revision		Revision as of 21:45, 31 August 2014
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	Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>		Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>

	~~===~~ [[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]]		[[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]]

	Safety information ===
			=== Safety information ===

	The chemicals in the DMSO+NaCl preservative are quite benign, being mostly water and table salt (see above). However, this information accompanies the two main chemicals.<br>1. DMSO (liquid, 20% of preservative by volume). "Causes skin irritation. Causes digestive and respiratory irritation. May cause severe eye irritation and possibly injury. May be absorbed through the skin. For eye contact, flush with water for 15 minutes and get medical aid immediately. For skin contact, flush with water and get medical aid. If ingested, do not induce vomiting and get medical aid immediately. If inhaled, remove to fresh air and get medical aid immediately."<br>2. EDTA (powder, @ 0.25M in preservative). "May cause irritation. For eye contact, flush with water and get medical aid. For skin contact, get medical aid if irritation occurs or persists. If ingested, give 2-4 cupfuls of milk or water and get medical aid. If inhaled, get medical aid if cough or other symptoms appear."		The chemicals in the DMSO+NaCl preservative are quite benign, being mostly water and table salt (see above). However, this information accompanies the two main chemicals.<br>1. DMSO (liquid, 20% of preservative by volume). "Causes skin irritation. Causes digestive and respiratory irritation. May cause severe eye irritation and possibly injury. May be absorbed through the skin. For eye contact, flush with water for 15 minutes and get medical aid immediately. For skin contact, flush with water and get medical aid. If ingested, do not induce vomiting and get medical aid immediately. If inhaled, remove to fresh air and get medical aid immediately."<br>2. EDTA (powder, @ 0.25M in preservative). "May cause irritation. For eye contact, flush with water and get medical aid. For skin contact, get medical aid if irritation occurs or persists. If ingested, give 2-4 cupfuls of milk or water and get medical aid. If inhaled, get medical aid if cough or other symptoms appear."

Mdawson at 04:45, 1 September 2014

2014-09-01T04:45:06Z

← Older revision		Revision as of 21:45, 31 August 2014
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	Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>		Both procedures A and B are designed to sample as much tissue and as little mesoglea (the 'jelly' of jellyfish) as possible. A third approach, not described above, is simply to preserve the whole animal, or a simple fraction thereof, if it has been cleared of potential contaminants and is small enough to fit easily into a vial with excess preservative (i.e. it is approximately <1 cm bell diameter). The figure below indicates the tissues best suited to sampling. The same methods also can be used to preserve coronate jellyfish and stauromedusae for DNA analyses.<br>

	=== [[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]]Safety information ===		=== [[Image:SampleTissues.jpg\|border\|left\|Sample Tissues]]

			Safety information ===

	The chemicals in the DMSO+NaCl preservative are quite benign, being mostly water and table salt (see above). However, this information accompanies the two main chemicals.<br>1. DMSO (liquid, 20% of preservative by volume). "Causes skin irritation. Causes digestive and respiratory irritation. May cause severe eye irritation and possibly injury. May be absorbed through the skin. For eye contact, flush with water for 15 minutes and get medical aid immediately. For skin contact, flush with water and get medical aid. If ingested, do not induce vomiting and get medical aid immediately. If inhaled, remove to fresh air and get medical aid immediately."<br>2. EDTA (powder, @ 0.25M in preservative). "May cause irritation. For eye contact, flush with water and get medical aid. For skin contact, get medical aid if irritation occurs or persists. If ingested, give 2-4 cupfuls of milk or water and get medical aid. If inhaled, get medical aid if cough or other symptoms appear."		The chemicals in the DMSO+NaCl preservative are quite benign, being mostly water and table salt (see above). However, this information accompanies the two main chemicals.<br>1. DMSO (liquid, 20% of preservative by volume). "Causes skin irritation. Causes digestive and respiratory irritation. May cause severe eye irritation and possibly injury. May be absorbed through the skin. For eye contact, flush with water for 15 minutes and get medical aid immediately. For skin contact, flush with water and get medical aid. If ingested, do not induce vomiting and get medical aid immediately. If inhaled, remove to fresh air and get medical aid immediately."<br>2. EDTA (powder, @ 0.25M in preservative). "May cause irritation. For eye contact, flush with water and get medical aid. For skin contact, get medical aid if irritation occurs or persists. If ingested, give 2-4 cupfuls of milk or water and get medical aid. If inhaled, get medical aid if cough or other symptoms appear."

AlfredoVB at 09:32, 14 March 2011

2011-03-14T09:32:15Z

← Older revision		Revision as of 02:32, 14 March 2011
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	=== Morphological Matrix  ===		=== Morphological Matrix  ===

	More Information<br>		[http://scyphozoan.ucmercedlibrary.info/wiki/Morphological_Matrix More Information]<br>

	=== Semaeostomeae ===		=== Semaeostomeae ===

AlfredoVB at 09:21, 14 March 2011

2011-03-14T09:21:00Z

← Older revision		Revision as of 02:21, 14 March 2011
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	=== Morphological Matrix  ===		=== Morphological Matrix  ===

	More Information		More Information<br>

	<br>

	=== Semaeostomeae ===		=== Semaeostomeae ===

AlfredoVB at 09:20, 14 March 2011

2011-03-14T09:20:41Z

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 === Morphological Matrix&nbsp;  ===
 More Information
-Species__________________		Date______________________
+<br>
 === Semaeostomeae  ===

AlfredoVB: /* Morphological Matrix */

2011-03-14T09:17:55Z

Morphological Matrix

← Older revision		Revision as of 02:17, 14 March 2011
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	=== Morphological Matrix  ===		=== Morphological Matrix  ===

			More Information

			Species__________________ Date______________________
			Recorder_______________ Cat.No.______________________

			PIC No. Description No. pics NOTES
			Tank
			1 Label
			2 Lateral view whole animal Front
			3 Lateral view whole animal Front RGB
			4 Lateral view whole animal Side
			5 whole animal from top RGB
			6 Close-up Front/Right Quadrant -Front or side
			7 Close-up Bell Margin -Front or side
			8 Close-up of tentacle -Front or side
			9 Close-up of Oral arms - Front or side
			10 Close-up of Oral Arm Filaments
			11 Close-up of terminal clubs - Front or side
			12 Close-up of Mouthlets - Front
			13 Close-up of Scapulae - Front or side
			14 Close-up of Scapular Filaments
			15 Close-up of Scapular Filaments RGB
			Lift up bell, drape oral arms to expose ends of manubrium
			16 Close-up of Manubrium/Mouth
			17 Oral side up from Top
			Table
			Subumbrella up, tentacles/oral arms out
			Measure Bell Diameter ______________
			Measure Bell thickness 1)____ 2)____ 3)____
			Measure Oral Disk _________________
			18 Whole medusae - RGB with flash
			19 Whole medusaer - Bottom Illum/B Background
			20 Whole medusae - Bottom Illum/Full Trans
			Move tentacles/oa from quadrant
			21 Close up of Quadrant - Bottom Illum/Full Trans
			22 Close-up of Quadrant - Bottom black
			23 Picture of center - Bottom Illum/B Background
			24 Picture of center - Bottom Illum/Full Trans
			25 Close-up of Velar Lappets - Bottom Illum/B Background
			26 Close-up of Velar Lappets - Bottom Illum/Full Trans
			27 Close-up of Rhopaliar Lappets - Bottom Illum/B Background
			28 Close-up of Rhopaliar Lappets - Bottom Illum/Full Trans
			29 Close-up of Rhopalium - Bottom Illum/B Background
			30 Close-up of Rhopalium - Bottom Illum/Full Trans
			31 Close-up of Coronal Muscles - Bottom Illum/B Background
			32 Close-up of Coronal Muscles - Bottom Illum/Full Trans
			33 Cloes-up of Radial Muscles - Bottom Illum/B Background
			34 Cloes-up of Radial Muscles - Bottom Illum/Full Trans
			35 Close-up of gonad - Bottom Illum/B Background
			36 Close-up of gonad - Bottom Illum/Full Trans
			Species__________________ Date______________________
			Recorder_______________ Cat.No.______________________

			PIC No. Description No. pics NOTES
			37 Close-up of canals 2 quads - Bottom Illum/Full Trans
			38 Close-up of canals 2 quads - Bottom Illum/B Blackground
			Move oral arms to top in a group - reveal oral pillars
			39 Close-up of Oral Pillar - Bottom Illum/B Background
			40 Close-up of Oral Pillar - Bottom Illum/Full Trans
			Measure depth of oral Pillar __________________________
			Measure height of oral pillar__________________________
			Measure the wide of oral pilar_________________________
			41 Close-up of Subumbrellar papillae - Bottom Illum/B Background
			42 Close-up of Subumbrellar papillae - Bottom Illum/Full Trans
			Splay 1/2 of oral arms and terminal clubs out
			43 Close-up of oral arms/terminal clubs - Bottom Illum/B Background
			44 Close-up of oral arms/terminal clubs - Bottom Illum/Full Trans
			Splay single oral arm out (winged portion esp.) - expose fenestrations
			45 Close-up of single oral arm - Bottom Illum/B Background
			46 Close-up of single oral arm - Bottom Illum/Full Trans
			Splay out scapulae as with oral arm (above)
			47 Close up of scapula - Bottom Illum/B Background
			48 Close up of scapula - Bottom Illum/Full Trans
			Pull up oral disk, take a look at subgenital porticus
			Record?______________________________
			Flip animal so exumbrella faces up
			49 Close-up of Exumbrella Quadrant - Bottom Illum/B Background
			50 Close-up of Exumbrella Quadrant - Bottom Illum/Full Trans
			51 Whole animal Exumbrella - Bottom Illum/B Background
			52 Whole animal Exumbrella - Bottom Illum/Full Trans
			If dying canals possible - flip animal and dye canals
			53 Close-up of dyed canals 2 quads
			Dissect Rhopalia - place in vial
			Remove tentacle(s) - place in vial

	=== Semaeostomeae ===		=== Semaeostomeae ===