Shipping information and details - Revision history

Mithril: +category

2011-01-04T08:29:03Z

+category

← Older revision		Revision as of 01:29, 4 January 2011
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	Jellyfish samples are necessarily preserved in 2-4% formalin (for morhpological analyses) and 95% ethanol (for DNA analyses*). Both formalin and ethanol are hazardous substances and therefore must be shipped with appropriate precaution. If shipping samples in jars full of preservative, the only way to ship them is to use couriers such as FedEx, [[www.dhl-usa.com/custserv/dangerousgoods.asp\|DHL]], and [http://www.ups.com/content/us/en/resources/ship/hazardous/index.html?WT.svl=SubNav UPS] which will deal with the packages as hazardous materials. Obviously, this is very expensive because [1] such packages are large and/or heavy due to all the preservative, [2] they receive special handling, and [3] it's a door-to-door service. Fortunately, it is usually possible to send samples preserved in 4% formalin or 95% ethanol through the regular postal service as long as their rules are followed ([http://www.usps.com/aviationsecurity USPS]). In my experience, most clerks at the post office are not familiar with the regulations regarding sending such substances through the mail (the usual response is that you cannot do it at all), so it is best to read the regulations yourself; usually you'll find that it's OK to send very small quantities if they're packaged accordingly. A method for packing and shipping samples that I learned from Lori J. Bell (and which she had learned from someone else) is explained below. We have never had any problem with this method.		Jellyfish samples are necessarily preserved in 2-4% formalin (for morhpological analyses) and 95% ethanol (for DNA analyses*). Both formalin and ethanol are hazardous substances and therefore must be shipped with appropriate precaution. If shipping samples in jars full of preservative, the only way to ship them is to use couriers such as FedEx, [[www.dhl-usa.com/custserv/dangerousgoods.asp\|DHL]], and [http://www.ups.com/content/us/en/resources/ship/hazardous/index.html?WT.svl=SubNav UPS] which will deal with the packages as hazardous materials. Obviously, this is very expensive because [1] such packages are large and/or heavy due to all the preservative, [2] they receive special handling, and [3] it's a door-to-door service. Fortunately, it is usually possible to send samples preserved in 4% formalin or 95% ethanol through the regular postal service as long as their rules are followed ([http://www.usps.com/aviationsecurity USPS]). In my experience, most clerks at the post office are not familiar with the regulations regarding sending such substances through the mail (the usual response is that you cannot do it at all), so it is best to read the regulations yourself; usually you'll find that it's OK to send very small quantities if they're packaged accordingly. A method for packing and shipping samples that I learned from Lori J. Bell (and which she had learned from someone else) is explained below. We have never had any problem with this method.

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	<br>'''Dr. Michael Dawson<br>School of Natural Sciences<br>University of California at Merced<br>5200 North Lake Road<br>Merced, CA 95340 USA'''<br>		<br>'''Dr. Michael Dawson<br>School of Natural Sciences<br>University of California at Merced<br>5200 North Lake Road<br>Merced, CA 95340 USA'''<br>

			[[Category:Identification]]

Mdawson at 06:09, 2 June 2010

2010-06-02T06:09:16Z

← Older revision		Revision as of 23:09, 1 June 2010
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	~~Please send all samples through airmail at room temperature to:~~

	~~<br>'''Dr. Michael Dawson<br>School of Natural Sciences<br>University of California at Merced<br>5200 North Lake Road<br>Merced, CA 95340 USA'''<br>~~

	~~<br>~~

	Jellyfish samples are necessarily preserved in 2-4% formalin (for morhpological analyses) and 95% ethanol (for DNA analyses*). Both formalin and ethanol are hazardous substances and therefore must be shipped with appropriate precaution. If shipping samples in jars full of preservative, the only way to ship them is to use couriers such as FedEx, [[www.dhl-usa.com/custserv/dangerousgoods.asp\|DHL]], and [http://www.ups.com/content/us/en/resources/ship/hazardous/index.html?WT.svl=SubNav UPS] which will deal with the packages as hazardous materials. Obviously, this is very expensive because [1] such packages are large and/or heavy due to all the preservative, [2] they receive special handling, and [3] it's a door-to-door service. Fortunately, it is usually possible to send samples preserved in 4% formalin or 95% ethanol through the regular postal service as long as their rules are followed ([http://www.usps.com/aviationsecurity USPS]). In my experience, most clerks at the post office are not familiar with the regulations regarding sending such substances through the mail (the usual response is that you cannot do it at all), so it is best to read the regulations yourself; usually you'll find that it's OK to send very small quantities if they're packaged accordingly. A method for packing and shipping samples that I learned from Lori J. Bell (and which she had learned from someone else) is explained below. We have never had any problem with this method.		Jellyfish samples are necessarily preserved in 2-4% formalin (for morhpological analyses) and 95% ethanol (for DNA analyses*). Both formalin and ethanol are hazardous substances and therefore must be shipped with appropriate precaution. If shipping samples in jars full of preservative, the only way to ship them is to use couriers such as FedEx, [[www.dhl-usa.com/custserv/dangerousgoods.asp\|DHL]], and [http://www.ups.com/content/us/en/resources/ship/hazardous/index.html?WT.svl=SubNav UPS] which will deal with the packages as hazardous materials. Obviously, this is very expensive because [1] such packages are large and/or heavy due to all the preservative, [2] they receive special handling, and [3] it's a door-to-door service. Fortunately, it is usually possible to send samples preserved in 4% formalin or 95% ethanol through the regular postal service as long as their rules are followed ([http://www.usps.com/aviationsecurity USPS]). In my experience, most clerks at the post office are not familiar with the regulations regarding sending such substances through the mail (the usual response is that you cannot do it at all), so it is best to read the regulations yourself; usually you'll find that it's OK to send very small quantities if they're packaged accordingly. A method for packing and shipping samples that I learned from Lori J. Bell (and which she had learned from someone else) is explained below. We have never had any problem with this method.
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	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)

			<br>

			Please send all samples through airmail at room temperature to:

			<br>'''Dr. Michael Dawson<br>School of Natural Sciences<br>University of California at Merced<br>5200 North Lake Road<br>Merced, CA 95340 USA'''<br>

Mdawson: /* Shipping tissues preserved in ethanol for DNA analysis. */

2010-06-02T06:08:27Z

Shipping tissues preserved in ethanol for DNA analysis.

← Older revision		Revision as of 23:08, 1 June 2010
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	Procedure: <br>		Procedure: <br>

	#Make sure the cap is screwed on firmly, with a label. Be sure to write the label in waterproof paper and pencil (Ethanol ~~rub out~~ the ink of permanent markers).<br>		#Make sure the cap is screwed on firmly, with a label. Be sure to write the label in waterproof paper and pencil (Ethanol will dissolve the ink of permanent markers).<br>
	#Place into the whirl-pak bag or box. Vials should be wrapped in small groups and wrapped with packaging tape. This makes one single unit out of many and will prevent movement and collision during ~~shippingseal~~ the bag.<br>		#Place into the whirl-pak bag or box. Vials should be wrapped in small groups and wrapped with packaging tape. This makes one single unit out of many and will prevent movement and collision during shipping, seal the bag.<br>
	#Put into the 4-mil plastic bag including the paper towel, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with 2 rubber bands.<br>		#Put into the 4-mil plastic bag including the paper towel, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with 2 rubber bands.<br>
	#Double ~~page~~ the vials or box with zip lock bags.<br>		#Double wrap the vials or box with zip lock bags.<br>
	#Place in the padded envelope along with the letter and copies of permits. <br>		#Place in the padded envelope along with the letter and copies of permits. <br>
	#Seal, label, and airmail it.<br>		#Seal, label, and airmail it.<br>
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	'''NOTES'''<br>		'''NOTES'''<br>

	The shipments of samples preserved in ethanol are regulated by the Department of Transportation. Shipping ethanol in limited quantities is allowed in almost all the commercial ~~curiers~~ following the next regulations:<br>1. Each pack of vials cannot exceed 1 liter each.<br>2. Shipment must be packed in strong, tight and secure containers.<br>3. Shipments must contain enough absorbent material (pads, cotton, etc.).<br>4. Total package weight cannot exceed 30 kg.<br>5. Shipping label:'''Limited quantity, ethanol, 3, UN1170, PG II. '''<br><br>		The shipments of samples preserved in ethanol are regulated by the Department of Transportation. Shipping ethanol in limited quantities is allowed in almost all the commercial couriers following the next regulations:<br>1. Each pack of vials cannot exceed 1 liter each.<br>2. Shipment must be packed in strong, tight and secure containers.<br>3. Shipments must contain enough absorbent material (pads, cotton, etc.).<br>4. Total package weight cannot exceed 30 kg.<br>5. Shipping label:'''Limited quantity, ethanol, 3, UN1170, PG II. '''<br><br>

	<br>		<br>

Lgomezdaglio: /* Shipping tissues preserved in formalin for morphological analyses. */

2010-06-01T07:15:15Z

Shipping tissues preserved in formalin for morphological analyses.

← Older revision		Revision as of 00:15, 1 June 2010
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	#Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>		#Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
	#Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>		#Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
	<br> <gallery widths=150px perrow=7>		<br> <gallery widths=150px perrow=4>
	Image:Pack1.jpg		Image:Pack1.jpg \|Drain the remain of formalin
	Image:Pack2.jpg		Image:Pack2.jpg \|Place a piece of cotton on the bottom or side of the bag
	Image:Pack3.jpg		Image:Pack3.jpg \|Remove air
	Image:Pack4.jpg		Image:Pack4.jpg \|Seal the bag
	Image:Pack5.jpg		Image:Pack5.jpg \|Use a double bag
	</gallery>		</gallery>

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	#Seal the box, label, and airmail it.		#Seal the box, label, and airmail it.
	<br>		<br>
	<gallery widths=150px perrow=7>		<gallery widths=150px perrow=4>
	Image:Pack6.jpg		Image:Pack6.jpg \|Place heaviest and bigger specimens in the bottom.
	Image:Pack7.jpg		Image:Pack7.jpg \|Fill the box with peanuts
	Image:Pack8.jpg		Image:Pack8.jpg \|Cardboard box ......
	Image:Pack9.jpg		Image:Pack9.jpg \|Use a double cardboard box
	</gallery>		</gallery>

Lgomezdaglio at 06:48, 1 June 2010

2010-06-01T06:48:09Z

← Older revision		Revision as of 23:48, 31 May 2010
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	#Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>		#Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
	#Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>		#Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
			<br> <gallery widths=150px perrow=7>
			Image:Pack1.jpg
			Image:Pack2.jpg
			Image:Pack3.jpg
			Image:Pack4.jpg
			Image:Pack5.jpg
			</gallery>

	#Put a layer of peanuts on the bottom of the smaller box, put the bag(s) in the box, placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. and fill with peanuts. <br>		#Put a layer of peanuts on the bottom of the smaller box, put the bag(s) in the box, placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. and fill with peanuts. <br>
	#Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. <br>		#Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. <br>
	#Place the letter and copies of permits on top of the peanuts. <br>		#Place the letter and copies of permits on top of the peanuts. <br>
	#Seal the box, label, and airmail it.		#Seal the box, label, and airmail it.
			<br>
			<gallery widths=150px perrow=7>
			Image:Pack6.jpg
			Image:Pack7.jpg
			Image:Pack8.jpg
			Image:Pack9.jpg
			</gallery>


	~~<br>~~

	<br>		<br>

	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)

Lgomezdaglio at 06:45, 1 June 2010

2010-06-01T06:45:13Z

← Older revision		Revision as of 23:45, 31 May 2010
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	#Carefully transfer the specimen into the whirl-pak bag if it was storage in jar. For speciemns in plastic bags, drain all the formalin.<br>		#Carefully transfer the specimen into the whirl-pak bag if it was storage in jar. For speciemns in plastic bags, drain all the formalin.<br>
	#Place a pad of cotton moisturized with tap water in the bottom of the bag.		#Place a pad of cotton moisturized with tap water in the bottom of the bag.
	#Carefully remove the air  from the whirl-pak bag without damaging the specimen, and seal the bag as was explained in the preservation of medusae section. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.		#Carefully remove the air  from the whirl-pak bag without damaging the specimen, and seal the bag as was explained in the [http://scyphozoan.ucmercedlibrary.info/wiki/Methods#Preservation_of_Medusae.C2.A0 preservation of medusae section]. <br>
			#Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
			#Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
			#Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. <br>
			#Put a layer of peanuts on the bottom of the smaller box, put the bag(s) in the box, placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. and fill with peanuts. <br>
			#Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. <br>
			#Place the letter and copies of permits on top of the peanuts. <br>
			#Seal the box, label, and airmail it.

	1. , seal the bag using a rubber band (link to preservation). 2. The organisms in the bag must have some air in there to avoid damage. Always use a double bag . All the bagged organisms must be placed in a cooler box or carton box (Fig. 7), placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. Use peanuts or any kind of paper to protect the samples. For carton boxes use a double carton box.		<br>

	<br>		<br>

	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)

Lgomezdaglio at 06:40, 1 June 2010

2010-06-01T06:40:05Z

← Older revision		Revision as of 23:40, 31 May 2010
Line 50:		Line 50:
	*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>		*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>

	Procedure: <br>		Procedure: <br>

	Jellyfish should be pack in plastic bags or plastic containers with tap water. FORMALIN is prohibited for almost all the worldwide courier companies, including post office and airlines cargo.		Jellyfish should be pack in plastic bags or plastic containers with tap water. FORMALIN is prohibited for almost all the worldwide courier companies, including post office and airlines cargo.

	#Carefully transfer the specimen into the whirl-pak bag if it was storage in jar. For speciemns in plastic bags, ~~Drain~~ all the formalin ~~and place~~ a pad of cotton moisturized with tap water in the bottom of the bag.~~<br>~~		#Carefully transfer the specimen into the whirl-pak bag if it was storage in jar. For speciemns in plastic bags, drain all the formalin.<br>
	#~~If there are more than a few ml of residual liquid in the bag. drain the bag.~~ Carefully remove ~~as much~~ air ~~as possible~~ from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.		#Place a pad of cotton moisturized with tap water in the bottom of the bag.
			#Carefully remove the air  from the whirl-pak bag without damaging the specimen, and seal the bag as was explained in the preservation of medusae section. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.

	1. , seal the bag using a rubber band (link to preservation). 2. The organisms in the bag must have some air in there to avoid damage. Always use a double bag . All the bagged organisms must be placed in a cooler box or carton box (Fig. 7), placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. Use peanuts or any kind of paper to protect the samples. For carton boxes use a double carton box.		1. , seal the bag using a rubber band (link to preservation). 2. The organisms in the bag must have some air in there to avoid damage. Always use a double bag . All the bagged organisms must be placed in a cooler box or carton box (Fig. 7), placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. Use peanuts or any kind of paper to protect the samples. For carton boxes use a double carton box.

Lgomezdaglio: /* Shipping tissues preserved in formalin for morphological analyses. */

2010-06-01T06:12:18Z

Shipping tissues preserved in formalin for morphological analyses.

← Older revision		Revision as of 23:12, 31 May 2010
Line 50:		Line 50:
	*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>		*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>

	Procedure:		Procedure: <br>

	#Carefully transfer the specimen into the whirl-pak bag. If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.		Jellyfish should be pack in plastic bags or plastic containers with tap water. FORMALIN is prohibited for almost all the worldwide courier companies, including post office and airlines cargo.

			#Carefully transfer the specimen into the whirl-pak bag if it was storage in jar. For speciemns in plastic bags, Drain all the formalin and place a pad of cotton moisturized with tap water in the bottom of the bag.<br>
			#If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.

			1. , seal the bag using a rubber band (link to preservation). 2. The organisms in the bag must have some air in there to avoid damage. Always use a double bag . All the bagged organisms must be placed in a cooler box or carton box (Fig. 7), placing the heaviest and bigger organisms at the bottom and the lighter and smaller on the top. Use peanuts or any kind of paper to protect the samples. For carton boxes use a double carton box.

	<br>		<br>

	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)

Lgomezdaglio at 06:59, 31 May 2010

2010-05-31T06:59:21Z

← Older revision		Revision as of 23:59, 30 May 2010
Line 42:		Line 42:
	Materials required:		Materials required:

	*preserved sample		*preserved sample
	*whirl-pak bag of appropriate size		*whirl-pak bag of appropriate size
	*three 30 cm x 60 cm '4 mil' plastic bags		*three 30 cm x 60 cm '4 mil' plastic bags
	*paper towel (or other absorbant material)		*paper towel (or other absorbant material)
	*rubber bands		*rubber bands
	*plenty of packing peanuts (not the corn ones that dissolve when wet) or other any other kind of protected material		*plenty of packing peanuts (not the corn ones that dissolve when wet) or other any other kind of protected material
	*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>		*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>

	Procedure:		Procedure:

	Carefully transfer the specimen into the whirl-pak bag. If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.		#Carefully transfer the specimen into the whirl-pak bag. If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.

	<br>		<br>

	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)

Lgomezdaglio at 06:23, 31 May 2010

2010-05-31T06:23:38Z

← Older revision		Revision as of 23:23, 30 May 2010
Line 40:		Line 40:
	== Shipping tissues preserved in formalin for morphological analyses. ==		== Shipping tissues preserved in formalin for morphological analyses. ==

	Materials required: preserved sample, whirl-pak bag of appropriate size, three 30 cm x 60 cm '4 mil' plastic bags, paper towel (or other absorbant material), 3 rubber bands, plenty of packing peanuts (not the corn ones that dissolve when wet), and two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box)		Materials required:

	Procedure: Carefully transfer the specimen into the whirl-pak bag. If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.		*preserved sample
			*whirl-pak bag of appropriate size
			*three 30 cm x 60 cm '4 mil' plastic bags
			*paper towel (or other absorbant material)
			*rubber bands
			*plenty of packing peanuts (not the corn ones that dissolve when wet) or other any other kind of protected material
			*two cardboard boxes (the smallest should have plenty of space for the specimens, and also fit comfortably inside the larger box) or styrofoam coolers<br>

			Procedure:

			Carefully transfer the specimen into the whirl-pak bag. If there are more than a few ml of residual liquid in the bag. drain the bag. Carefully remove as much air as possible from the whirl-pak bag without damaging the specimen, and seal the bag. Put the whirl-pak bag into one of the 4-mil plastic bags and include some of the paper towel. Twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Place this bag into the second of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Now place this bag into the third of the 4-mil plastic bags, include some of the paper towel, remove excess air, twist the neck of the plastic bag until it is tightly coiled, bend the coil back on itself, and secure with one of the rubber bands. Put a layer of peanuts on the bottom of the smaller box, put the bag in the box, and fill with peanuts. Seal the box. Put a layer of peanuts on the bottom of the larger box, put the smaller box in the larger box, and fill with peanuts. Place the letter and copies of permits on top of the peanuts. Seal the box, label, and airmail it.

	<br>		<br>

	*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)		*A relatively benign, good, alternative to 95% ethanol is DMSO+NaCl (see Dawson et al. 1998)